In collecting semen indigenous a stallion, a run mare or breeding phantom is typically used. Stallions deserve to be conveniently trained to mountain a reproduction phantom. Those trained to mountain a phantom do collection lot safer and an ext consistent. The phantom have to be design to be comfortable because that the stallion and should mimic the same angle together the mare. If a run mare is used, she have to be in an excellent standing heat and have she tail wrapped and perineal area washed. Restrain the mare to stop injury to the stallion or personnel assisting.

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An fabricated vagina is supplied to collect the semen because that evaluation. Several fabricated vaginas are available: the Colorado, Missouri and also Japanese models. Selection of an synthetic vagina (AV) is identified mainly through management and stallion preference. Come prepare the synthetic vagina, fill it with water come obtain ideal temperature and also pressure. Some models usage a mix of water and air to obtain the suitable pressure. Due to the fact that of stallions’ individual natures, water temperature preference, pressure and also lubrication will certainly vary. Therefore, the is important to prepare the AV to the an individual preference for the stallion gift collected.

Colorado AV

Water temperature because that the AV is normally in between 45 come 48°C. However, water temperature have to be administer by the ideal repertoire temperature for an individual stallion. Avoid greater temperatures to protect against irritation to the stallion’s penis and also sperm damage. Lubricate the AV v a sterile, non-spermicidal lubricant just before use. Carry out not use products detrimental to sperm cells. Use enough lubricant to allow easy penetration with little resistance. Warm the collection bottle and keep it warm during collection to prevent damaging sperm cells.

The stallion must be introduced to the mare and also stimulated come let down. ~ above stimulation, to wash the stallion v clean warmth water. Perform not usage soap or disinfectant to to wash the stallion’s penis. The arsenal area need to be big enough to stop injury to all those involved. When the stallion has actually reached complete erection, enable him to mount the phantom or mare because that collection. The stallion’s penis should be deflected right into the AV, not grasped, since some stallions take violation to being grasped. The AV should be held firmly for the stallion come thrust versus and have to be at the very same angle as the mare’s vagina. As the stallion ejaculates, the AV need to be lowered to permit the semen to run right into the collection bottle. Ejaculation deserve to be determined by watching because that the “flagging” movement of the stallion’s tail or through the pulsation of semen passing v the urethra at the basic of the penis. ~ above completion, drainpipe the water come let the staying semen drainpipe into the collection bottle. Then store the semen in an incubator till evaluated.

Care throughout Collection

Great care is taken during collection come ensure the spermatozoa room in an setting that guards versus cold shock and contamination of spermicidal agents. For most stallions, A.V.’s are generally prepared because that inner temperatures between 100.0 to 112.0o Fahrenheit (38 to 45o C) at the moment of collection. Stallions space sensitive to pressure and also temperature changes, and accurate security is essential for fostering sex drive.

Non-spermicidal lubricants room used, and spermicides such as water and light room not allowed to come in call with the ejaculate. Any equipment or materials that come in contact with the spermatozoa room warmed to around 100.0o Fahrenheit (38o C). As soon as extended, semen is housed in an incubator in ~ 100.0o Fahrenheit (38o C) until inseminated. Insemination need to follow within the hour or as conveniently as feasible since the longer semen is stored, the better the ns of live, motile spermatozoa cells.

Semen write-up Collection

Recent transforms in several major breed registries have enabled for boosted use of cooled and frozen semen. Cooling or freeze semen permits for semen to stay viable longer. As such, semen deserve to be shipped to mares, remove the cost and also stress the shipping a mare and foal. Freshly collected extended semen deserve to be cooled come 40.0o Fahrenheit, transported in containers specially design to preserve the cool temperature, and inseminated in mares 24 come 48 hours after collection. The success of using cooled semen relies on how well a details stallion’s spermatozoa respond come cooling, the synchronization that the mare’s estrus to the time of insemination, and also how well appropriate semen collection and also handling techniques are followed.

Although frozen semen is routinely supplied in other varieties of livestock, it has not been as well researched in because of each other registry limitations on its use. However, it will become an ext common because of recent changes adopted by many registries because that legalization that its use. Frozen semen is packaged in pellets, plastic straws or glass ampules and stored in liquid nitrogen in ~ temperatures below -320.0o Fahrenheit.

Semen arsenal schedules

Collection through a jump mare.

Collecting or reproduction once every week to reduce the weekly sperm output compared with breeding or collecting everyday or every other day. Sperm output per mainly is similar whether collecting every other day or daily. Therefore, for maximum quantities of sperm collection and also minimum labor, stallions must be accumulated every various other day. The every-other-day schedule is usual for ranches using synthetic insemination.

Managers breeding stallions by natural cover might need them to cover mares much more frequently than every various other day. Much more frequent repertoire intervals will certainly not an outcome in a far-reaching increase in weekly sperm output. Rather, individual collections will have fewer spermatozoa. Even if it is or no these to reduce are significant enough come lessen fertility when reproduction every work or twice everyday will depend on the stallion’s spermatozoa production capability.

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The stallion’s sex drive and his ability to develop spermatozoa will certainly place boundaries on how regularly he can efficiently breed mares. Sex drive, as monitored through reaction time and number of mounts necessary before ejaculation, generally will no be affected by boosting the breeding frequency native every various other day to daily collections. However, managers must treat stallions as individuals and also regulate collection intervals appropriately. Overuse can reason decreases in sex drive, it was observed by the stallion’s disinterest in breeding, much longer mounting times required before breeding, or absence of ejaculation throughout breeding.